Antibiotic Resistance Bacteria in Tertiary Hospitals in Chittagong, Bangladesh

Abstract

Nosocomial infections (HAI) are major cause for mortality and morbidity worldwide. In low income countries, data suggests 6.5% - 33% of patients have HAI with pneumonia being the most frequent..Antibiotic resistance is highly prevalent in developing countries due to self medication, easy availability and poor regulatory controls. Clinicians have been left with limited antibiotic drug options for the treatment of bacterial infections due to escalated rates of resistance. This comparative study aimed to identify microorganisms from hospital surfaces in two major tertiary care hospitals in Chittagong, Bangladesh. It also identifies antibiotic susceptibility of the samples to antibiotics commonly used in Bangladesh. Samples were collected by swabbing different environmental surface around patients in both hospitals. Identification of bacteria was done by culturing in nutrient media and various common biochemical techniques. Antibiotic sensitivity was determined by disk diffusion method. During the study, 27 samples were collected from different surfaces in different wards of the hospitals. The predominating organisms were Streptococcus, Staphylococcus, Bacillus, Pseudomonas and Serratia. The isolates of organisms showed high level of resistance to commonly used antibiotics especially a fourth generation cephalosporin, cefepime. In addition, antibiotic sensitivity tests showed small colonies or film of growth within zone of inhibition of some of the samples known as “satellite colonies”. The study identified bacterial isolates responsible for HAI in tertiary hospitals and their susceptibility to antibiotics. Further research is currently being conducted on understanding the satellite colonies some of the isolates from hospital surface swabs have exhibited.