Extraction and Purification of Alpha Amylase from Bacillus amyloliquefaciens and Identification of the Strain by 16s rRNA Gene Sequencing

Abstract

In the present study, alpha amylase was produced from Bacillus amyloliquefaciens in optimized condition by submerged fermentation technique. Biochemical tests were performed to identify the bacteria. The enzyme was extracted and purified by ammonium sulphate precipitation. Amylase activity and specific activity of the purified amylase was found to be 10 U/ml/min and 0.89 U/mg/min respectively. The enzyme was further purified by dialysis and size exclusion chromatography. The 16s rRNA gene was extracted from the bacteria and sent for sequencing to identify the strain. The result of the sequence analysis of the gene will be used to design primer and clone the amylase gene to over express the enzyme for industrial use in textile industry.