Comparative genomics of Mycobacterium reveals evolutionary trends of M. avium complex

Abstract

Mycobacterium is gram positive, slow growing, disease causing Actinobacteria. Beside potential pathogenic spe- cies, Mycobacterium also contains opportunistic pathogens as well as free living non-pathogenic species. Disease

related various analyses on Mycobacterium tuberculosis are very widespread. However, genomic study of overall Mycobacterium species for understanding the selection pressure on genes as well as evolution of the organism is still illusive. MLSA and 16s rDNA based analysis has been generated for 241 Mycobacterium strains and a detailed analysis of codon and amino acid usage bias of mycobacterial genes, their functional analysis have been done. Further the evolutionary features of M. avium complex also have been revealed. Mycobacterial genes are moderately GC rich showed higher expression level in PPs and significant negative correlation with biosynthetic

cost of proteins. Translational selection pressure was observed in mycobacterial genes. MAC showed close re- lationship with NPs and higher evolutionary rate in MAC revealed their constant evolving nature.