Green Synthesis of Silver Nanoparticles using the Leaf Extract of Filicium decipiens and its Anti-Microbial Activity

Abstract

Silver nanoparticles has been used since ages; even now it is used in almost all areas like medicine, textiles, industries, cosmetics, purification and dying. There are many approaches to synthesize silver nanoparticles. However, these approaches are either harmful to the environment or highly expensive. An attempt has been made in this eco-friendly approach

towards the green synthesis of silver nanoparticles (AgNPs) using the leaves of Filicium decipiens. Characterization of as- synthesized AgNPs were done using UV-Visible spectroscopy and Fluorescence spectroscopy which confirmed the formation

of AgNPs. Scanning Electron Microscope (SEM) confirmed its rounded shape and X-ray diffraction (XRD) determined its crystalline nature as face centered cubic structure. Furthermore, Dynamic Light Scattering (DLS) was also done in order to know the average diameter and zeta potential of AgNPs. However, it did not show potential results due to the aggregates formed during the green synthesis of AgNPs. In addition to this, anti-microbial test against bacteria such as gram negative

(Escherichia Coli) and gram positive (Bacillus subtilis) were done using well-diffusion method and also its application of anti- microbial activity was tested over fabric to understand its application in textile industries. In both the cases, AgNPs showed

more efficiency in gram negative than gram-positive bacteria.